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Enzyme Substrates · Enzyme substrate (research reagent) · CASP-095

Ac-IETD-AFC

Research use · ships internationally
$85.00 / 1mg vial
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Ac-IETD-AFC reference vial
Drug class
Enzyme substrate (research reagent)
Status
Research use only
Catalog code
CASP-095
Also known as
CASP-095
Reviewed
2026-06-01 · Kindr Health Clinical Team

What Ac-IETD-AFC is studied for

  • Kinetic (kcat / Km) characterization of proteases and hydrolases
  • High-throughput inhibitor screening
  • Selectivity profiling across related enzyme families
  • Diagnostic and academic activity assays

Mechanism of action

Ac-IETD-AFC sits within the Enzyme Substrates research class (CPC reference CASP-095). The peptide encodes a recognition sequence flanked by a reporter group (pNA, AMC, MCA, FAM/DABCYL). Enzyme cleavage releases the reporter, producing a quantifiable optical signal proportional to catalytic activity. Investigators typically incorporate Ac-IETD-AFC as a reference standard, tool ligand, or substrate in the assay contexts listed above. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).

Frequently asked questions

What is Ac-IETD-AFC?

Ac-IETD-AFC (CPC catalog code CASP-095) is a synthetic enzyme substrate (research reagent) in the enzyme substrates category. It is provided as a lyophilized ~1 mg vial for in-vitro research use only.

What is Ac-IETD-AFC studied for?

Published and internal research programs use Ac-IETD-AFC as a reference standard in enzyme substrates workflows — for example, kinetic (kcat / km) characterization of proteases and hydrolases and high-throughput inhibitor screening. Human clinical evidence is not established.

Is Ac-IETD-AFC FDA-approved for human use?

No. Ac-IETD-AFC is a research-grade reference peptide and is not approved by the FDA or any national regulator for human or veterinary therapeutic use. It is offered exclusively for in-vitro laboratory research and does not ship to the United States for consumer use.

How is Ac-IETD-AFC typically used in the lab?

In a enzyme substrates workflow, Ac-IETD-AFC is reconstituted in sterile buffer or DMSO (solubility-dependent) and applied in the researcher's chosen assay format. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).

How should Ac-IETD-AFC be stored?

Store lyophilized at −20 °C, protected from light. DMSO stocks stable at −80 °C for months. Follow your institution's standard operating procedures for handling synthetic peptides.

Where does the Ac-IETD-AFC product page point to?

This kindr reference page mirrors CPC Scientific's catalog metadata for CASP-095. The upstream reference sheet — including sequence, purity, MW, and application notes where published — is linked from the buy panel. All orders placed on kindr Peptides ship internationally under research-use terms only.

What quality specifications apply to Ac-IETD-AFC?

Research-grade peptides in the CPC catalog are synthesized by solid-phase peptide synthesis (SPPS), purified by preparative RP-HPLC, and characterized by analytical HPLC and mass spectrometry. Typical purity is ≥95%; certificate of analysis is available on request.

Internal reference sheetOpen the Ac-IETD-AFC physicochemical & handling reference →Upstream reference sheetView CPC Scientific catalog page for CASP-095 →Add to cart · International research catalogBuy Ac-IETD-AFC in the kindr Peptides Shop →

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Research & medical disclosure. This page is educational reference content reviewed by a licensed physician. It is not medical advice and does not establish a clinician–patient relationship. Peptides offered on the kindr Peptides Shop are sold for in vitro laboratory research purposes only and ship worldwide, including the United States. For U.S. clinical care, use the kindr Longevity intake.