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Peptides shop · MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) · Reference sheet

MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate)

Reference sheet · Protease Substrates · CPC catalog code MMPS-014

Identity

Name
MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate)
Catalog code
MMPS-014
Category
Protease Substrates

Physicochemical properties

Sequence
DNP-Pro-Leu-Ala-Tyr-Trp-Ala-Arg-OH (trifluoroacetate salt)
One-letter
PLAYWAR
Residues
7 amino acids
Modifications
DNP
Net charge (pH 7)
+1 (1 basic / 0 acidic)
Hydrophobic residues
86%
Cysteine present
No

This sequence carries terminal or reporter modifications (DNP), so no molecular formula or weight is published here — the modified mass must come from the lot certificate of analysis. Residue composition and charge describe the peptide backbone only. Composition figures above are derived arithmetically from the published sequence for the unmodified free peptide. Salt forms (commonly trifluoroacetate or acetate), amidation, and other modifications shift the observed mass — always reconcile against the current lot certificate of analysis.

Reconstitution reference

Volumes for a 1 mg vial of MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) at common working concentrations. Add solvent slowly down the vial wall and swirl — do not vortex or shake.

10 mg/mL
0.1 mL diluent per 1 mg
5 mg/mL
0.2 mL diluent per 1 mg
2 mg/mL
0.5 mL diluent per 1 mg
1 mg/mL
1 mL diluent per 1 mg

Handling, storage & reconstitution

Upstream storage
-20 ± 5 °C

General guidance for this class

Reference standards in the Protease Substrates class are typically shipped as lyophilized powder and stored desiccated at −20 °C protected from light. Once reconstituted in bacteriostatic water or the assay-appropriate buffer, aliquot and store at −80 °C to minimize freeze–thaw cycles. Confirm handling requirements against your institutional biosafety and radiation protocols before use.

Mechanism & research applications

MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) sits within the Protease Substrates research class (CPC reference MMPS-014). The peptide encodes a specific protease-recognition motif flanked by a reporter (pNA, AMC, MCA / Dnp FRET pair). Enzymatic cleavage releases the reporter, producing signal directly proportional to catalytic activity. Investigators typically incorporate MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) as a reference standard, tool ligand, or substrate in the assay contexts listed above. Read on kinetic plate readers at the reporter-appropriate wavelength.

Studied for

  • Kinetic (kcat / Km) protease characterization
  • Inhibitor screening and IC₅₀ determination
  • Selectivity profiling across protease families
  • Cell-based and diagnostic activity assays

Frequently asked

What is MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate)?

MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) (CPC catalog code MMPS-014) is a synthetic protease substrate (research reagent) in the protease substrates category. It is provided as a lyophilized ~1 mg vial for in-vitro research use only.

What is MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) studied for?

Published and internal research programs use MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) as a reference standard in protease substrates workflows — for example, kinetic (kcat / km) protease characterization and inhibitor screening and ic₅₀ determination. Human clinical evidence is not established.

Is MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) FDA-approved for human use?

No. MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) is a research-grade reference peptide and is not approved by the FDA or any national regulator for human or veterinary therapeutic use. It is offered exclusively for in-vitro laboratory research and does not ship to the United States for consumer use.

How is MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) typically used in the lab?

In a protease substrates workflow, MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) is reconstituted in sterile buffer or DMSO (solubility-dependent) and applied in the researcher's chosen assay format. Read on kinetic plate readers at the reporter-appropriate wavelength.

Sources

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Related peptides in Protease Substrates

Other reference standards in the same class — commonly cross-referenced during assay design and comparator selection.

MMP-2/MMP-9 Substrate I, Fluorogenic
MMP-1/MMP-9 Substrate, Fluorogenic
MMP-2/MMP-7 Substrate, Fluorogenic
MMP-1 Substrate I, Fluorogenic
MMP-3 Substrate I, Fluorogenic
MMP-2 Substrate, Fluorogenic
MMP Substrate V, Fluorogenic
MMP Substrate, Fluorogenic
Research use only. The peptides referenced on this page are supplied to qualified laboratories for in-vitro or non-human research. They are not drugs, foods, cosmetics, or dietary supplements, are not for human consumption, and are not intended to diagnose, treat, cure, or prevent any disease. Physicochemical values shown here are transcribed from the cited upstream reference sheet(s); confirm with your own certificate of analysis before use.