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Reference sheet · Enzyme Substrates · CPC catalog code CASP-046
This sequence carries terminal or reporter modifications (Z, pNA), so no molecular formula or weight is published here — the modified mass must come from the lot certificate of analysis. Residue composition and charge describe the peptide backbone only. Composition figures above are derived arithmetically from the published sequence for the unmodified free peptide. Salt forms (commonly trifluoroacetate or acetate), amidation, and other modifications shift the observed mass — always reconcile against the current lot certificate of analysis.
Volumes for a 1 mg vial of Z-Val-Asp-Val-Ala-Asp-pNA at common working concentrations. Add solvent slowly down the vial wall and swirl — do not vortex or shake.
Reference standards in the Enzyme Substrates class are typically shipped as lyophilized powder and stored desiccated at −20 °C protected from light. Once reconstituted in bacteriostatic water or the assay-appropriate buffer, aliquot and store at −80 °C to minimize freeze–thaw cycles. Confirm handling requirements against your institutional biosafety and radiation protocols before use.
Z-Val-Asp-Val-Ala-Asp-pNA sits within the Enzyme Substrates research class (CPC reference CASP-046). The peptide encodes a recognition sequence flanked by a reporter group (pNA, AMC, MCA, FAM/DABCYL). Enzyme cleavage releases the reporter, producing a quantifiable optical signal proportional to catalytic activity. Investigators typically incorporate Z-Val-Asp-Val-Ala-Asp-pNA as a reference standard, tool ligand, or substrate in the assay contexts listed above. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).
Z-Val-Asp-Val-Ala-Asp-pNA (CPC catalog code CASP-046) is a synthetic enzyme substrate (research reagent) in the enzyme substrates category. It is provided as a lyophilized ~1 mg vial for in-vitro research use only.
Published and internal research programs use Z-Val-Asp-Val-Ala-Asp-pNA as a reference standard in enzyme substrates workflows — for example, kinetic (kcat / km) characterization of proteases and hydrolases and high-throughput inhibitor screening. Human clinical evidence is not established.
No. Z-Val-Asp-Val-Ala-Asp-pNA is a research-grade reference peptide and is not approved by the FDA or any national regulator for human or veterinary therapeutic use. It is offered exclusively for in-vitro laboratory research and does not ship to the United States for consumer use.
In a enzyme substrates workflow, Z-Val-Asp-Val-Ala-Asp-pNA is reconstituted in sterile buffer or DMSO (solubility-dependent) and applied in the researcher's chosen assay format. Read on kinetic plate readers with the reporter-appropriate filter set (e.g. 405 nm, 380/460 nm).
Other reference standards in the same class — commonly cross-referenced during assay design and comparator selection.